Methods: The experiments of LF uptake by BeWo cells were performed after preincubation and in the presence of the P-glycoprotein inhibitors (Cyclosporin A, Verapamil and Quercetin), the organic anion/cation transporter inhibitor (Cimetidine) and the MCT substrates (lactic acid and salicylic acid).
Results: P-glycoprotein inhibitors increased the uptake of LF by BeWo cells. The increase in LF accumulation by Cyclosporin A, Verapamil and Quercetin was by 30, 90 and 80%, respectively. Cimetidine, the organic cation inhibitor, increased the transport of LF by 48%. Lactic acid and salicylic acid, the MCT substrates, initially decreased the accumulation of LF by 30% and subsequently increased the uptake of LF by 500 and 53%, respectively.
Conclusions: The uptake of LF by human trophoblast cells is mediated by multiple transporters as well as passive diffusion.
Polachek H, Holcberg G, Polachek J, Rubin M, Feinshtein V, Sheiner E, Ben-Zvi Z.
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev Beer-Sheva, Israel
Placental transfer of Levofloxacin (LF), a broad spectrum fluoroquinolone antibiotic, and its inhibition was investigated in BeWo cells, a human trophoblast cell line.